Characterization of γ-crystallins from eye lenses of shark: closer structural similarity to mammalian than other piscine γ-crystallins?
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منابع مشابه
Age-dependent association of γ-crystallins with aged α-crystallins from old bovine lens
PURPOSE Previous theoretical and experimental studies have predicted that the loss of weak protein interactions between alpha- and gamma-crystallins could result in a decrease in the transparent properties of the aging lens. METHODS alpha-Crystallins were prepared from the nucleus of old bovine lens, and gamma-crystallins were prepared from whole fetal bovine lens or from the nucleus of old b...
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Crystallins from pigeon eye lenses were isolated and purified by gel-permeation chromatography and characterized by gel electrophoresis, amino-acid composition and sequence analysis. Alpha- and beta-crystallins could be obtained in relatively pure forms by single-step size-exclusion chromatography whereas an extra step of ion-exchange chromatography was needed for the separation of delta-crysta...
متن کاملPrediction of Aggregation Sites in Human Eye Lens γ-Crystallins: Implications in Cataract
Cataract, a leading cause of blindness, occurs due to aggregation of crystallin proteins in eye lens. In this paper, the aggregation site in three major γ-crystallins of human eye lens has been identified unambiguously by using their primary sequences with the help of PASTA, Tango and AGGRESCAN. We have found that some hydrophobic residues in C-terminal domain of γ-crystallins play putative rol...
متن کاملComparative proteomics analysis of degenerative eye lenses of nocturnal rice eel and catfish as compared to diurnal zebrafish
PURPOSE The aim of this study was to determine the lens crystallin diversity of degenerative eyes from the rice eel (Monopterus albus) and walking catfish (Clarias batrachus) as compared to that of zebrafish (Danio rerio) by using comparative proteomics methodologies. We endeavored to investigate the evolution of vertebrate lenses particularly concerning the functional loss of lenses in degener...
متن کاملMolecular diversity and genomic organisation of the α, β and γ eye lens crystallins from the Antarctic toothfish Dissostichus mawsoni
The eye lens of the Antarctic toothfish living in the −2 °C Southern Ocean is cold-stable. To investigate the molecular basis of this cold stability, we isolated, cloned and sequenced 22 full length crystallin cDNAs. We found two α crystallins (αA, αB), six β crystallins (βA1, βA2, βA4, βB1, βB2, βB3) and 14 γ crystallins (γN, γS1, γS2, γM1, γM3, γM4, γM5, γM7, γM8a, γM8b, γM8c, γM8d, γM8e, and...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1989
ISSN: 0014-5793
DOI: 10.1016/0014-5793(89)80677-5